Brothers Joel and Joseph Holleman died and were buried in 1862, but their remains were unearthed in Raleigh last month by development. Excavation near PNC Arena turned up the brothers' bones.
On Saturday morning, the two men rested under honor guard at the North Carolina Museum of History.
A horse-drawn artillery caisson then bore them to Oakwood Cemetery where they were buried with a military and Masonic service by the 26th North Carolina Regiment.
The Sons of the Confederate Veterans also attended the burial.
Officials said Joseph Holleman enlisted with the 26th Regiment in September 1861 and died early the next year of pneumonia in Morehead City at age 22.
Joel Holleman was not a soldier, but officials say they think he worked at the state Fairgrounds Hospital in Raleigh and was listed by the federal government as a Confederate collaborator. He died at age 28.