The former first lady and the singer have been friends for years. They even teamed up in 2011 for Obama's "Let's Move" campaign, which aimed to fight childhood obesity.
Obama was spotted in the audience at the On The Run II performance alongside Beyoncé's mother, Tina Knowles Lawson.
And she wasn't the only Obama in the house as daughter Sasha was also photographed at the show.
Beyoncé performed at Barack Obama's inauguration ball in 2009 and sang the national anthem at his inauguration in 2013.
Mrs. Obama took their two daughters to see Jay and Bey in concert in Chicago in 2014. She also attended the Global Citizen Festival in 2015, where Beyoncé was one of the featured performers.